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陈龙

 

发布日期:2015-07-21  浏览次数:825

陈龙

陈龙  男,汉族,安徽安庆人,研究生学历,博士学位。南京师范大学教授、博士生导师。美国癌症协会(AACR)、中国生理学会、中国生化与分子生物学会、中国细 胞生物学会、中国病理生理学会、中国畜牧兽医学会会员。World J Gastroenterol、World J Exp Med、Open Vet Sci J等刊物编委。Acta Pharmacol Sin、Acupunct Med、Am J Physiol-Cell Ph、Brit J Pharmacol、Pharmacol Rep、Cancer Chemother Pharmacol、Cancer Invest、Cell Mol Neurobiol、Cytokine、IUBMB Life、Histol Histopathol、Hum Exp Toxicol、J Dairy Res、J Hazard Mater、J Leukoc Biol、J Pharm Pharmacol、Peptides、Pharm Biol、Vaccine等刊物审稿人。

学习和工作经历

1981-1985年  安徽农业大学动物医学系动物医学专业学士;1987-1988年  武汉大学生物化学系 访问学者;1991-1998  年南京农业大学动物医学院生理学与生物化学专业 硕士、博士;1997年  德国Hanover动物医学院 合作访问学者;2000-2001年  南京医科大学公共卫生学院江苏省应用毒理重点实验室 访问学者;2006-2008年  美国Louisiana州立大学卫生科学中心生化与分子生物学系博士后访问学者。

1985-1998年  安徽科技学院动物医学系助教、讲师、副教授,1998年8月  作为高层次人才引进南京师范大学生命科学学院,曾先后担任动物教研中心主任、生物学系主任、生命科学学院副院长等职务,2001年破格晋升为教授。现为生 命科学学院 生理学与生物化学教授,生化与分子生物学博士点学科研究方向带头人,生理学硕士点学科带头人。

研究方向

免疫网络信号分子及细胞粘附、迁移和凋亡的信号转导网络及其靶向调控。

研究内容和兴趣

从免疫生理学与生物化学、病理生理学角度揭示机体、组织、细胞损伤与抗损 伤保护的免疫学机制和分子机制——致力于细胞生存和死亡的信号转导网络研究,探索靶向药物调控分子机制。特别关注氧化应激、环境化学污染物镉影响人类健康 如大脑神经元凋亡、退化及其相关疾病的发生机理及调控和新药探索;神经-内分泌-免疫网络中的多信号分子的相互关系及调控策略,为指导有益免疫应答、避免 有害应答,寻找和开发新型免疫活性调节因子提供新思路和新方法;肿瘤发生中相关信号分子在其粘附、迁移中的作用,为寻找靶向抑制药物提供理论依据和指导。 具体包括:

  1. 神经细胞氧化应激介导mTOR抑制在帕金森病发生中作用分子机制;
  2. 人源性B淋巴细胞刺激因子(hsBAFF)信号转导及免疫调节机制;
  3. 环境污染物镉致神经细胞凋亡过程中MAPKs和Akt/mTOR通路信号转导网络及靶向调控机制;
  4. 肿瘤细胞粘附、迁移过程中mTOR信号及其相关分子的信号转导及靶向药物抑制作用的分子机制;
  5. 神经-内分泌-免疫网络中的多信号分子的相互关系及调控。

研究项目与成果

先后承担和主持国家自然科学基金、欧洲联盟资助的国际合作项目子课题、科技部“十五”科技专项子课题、江苏省科委“十五”科技攻关项目以及高校自然 科学重大基础研究项目等课题。已在Cancer Res、Oncogene、Oncotarget、Cell Mol Life Sci、Free Radic Biol Med、Carcinogenesis、Neuropharmacology、J Biol Chem、Biochem Pharmacol、Cell Cycle、Cell Signal、J Neurochem、Int J Biochem Cell Biol、Lab Invest、Neuropath Appl Neuro、Toxicol Sci、Vaccine、Cytokine等刊物发表论文160余篇,其中SCI收录45篇。申报发明专利授权2项。先后获江苏省南京市科技进步二等奖1 项、安徽省高校、江苏省南京市和农业部科技进步三等奖各1项、教育部科技进步自然科学奖二等奖2项。

ö 代表性研究论文*Corresponding author, 1co-first author)

  1. Xu C1, Zhang H1, Liu C, Zhu Y, Wang X, Gao W, Huang S*, Chen L*. Rapamycin inhibits Erk1/2-mediated neuronal apoptosis caused by cadmium. Oncotarget 2015; May 25. [Epub ahead of print] (SCI收录, IF= 6.359).
  2. Chen L*, Xu B, Liu L, Liu C, Luo Y, Chen X, Barzegar M, Chung J, Huang S*. Both mTORC1 and mTORC2 are involved in the regulation of cell adhesion. Oncotarget 2015; 6: 7136-7150 (SCI收录, IF=6.359).
  3. Zeng Q, Zhang H, Qin J, Xu Z, Gui L, Liu B, Liu C, Xu C, Liu W, Zhang S, Huang S*, Chen L*. Rapamycin inhibits BAFF-stimulated cell proliferation and survival by suppressing mTOR-mediated PP2A-Erk1/2 signaling pathway in normal and neoplastic B-lymphoid cells. Cell Mol Life Sci. 2015; DOI: 10.1007/s00018-015-1976-1 (SCI收录, IF=5.808).
  4. Xu C1, Liu C1, Liu L, Zhang R, Zhang H, Chen S, Luo Y, Chen L*, Huang S*. Rapamycin prevents cadmium-induced neuronal cell death via targeting both mTORC1 and mTORC2 pathways. Neuropharmacology 2015; 97: 35-45 (SCI收录, IF=5.106).
  5. Chen L, Liu C, Huang S. A deut of mTORC1/2 for cell adhesion. Cell Cycle 2015; 14: 1131-1132 (SCI收录, IF=4.565).
  6. Liu C1, Zhang R1, Sun C, Zhang H, Xu C, Liu W, Gao W, Huang S*, Chen L*. Resveratrol prevents cadmium activation of Erk1/2 and JNK pathways from neuronal cell death via protein phosphatases 2A and 5. J Neurochem. 2015; In Press (SCI收录, IF=4.281).
  7. Zhou Q1, Liu C1, Liu W1, Zhang H, Zhang R, Liu Jia, Zhang J, Xu C, Liu L, Huang S1, Chen L1,*. Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, leading to neuronal apoptosis. Toxicol Sci. 2015; 143; 81-96 (SCI收录, IF=3.854).
  8. Chen S1, Ren Q1, Zhang J, Ye Y, Zhang Z, Xu Y, Guo M, Ji H, Xu C, Gu C, Gao W, Huang S*, Chen L*. N-acetyl-L-cysteine protects against cadmium-induced neuronal apoptosis by inhibiting ROS-dependent activation of Akt/mTOR pathway in mouse brain. Neuropath Appl Neuro. 2014; 40: 759-777 (SCI收录, IF=4.97).
  9. Xu Y1, Liu C1, Chen S, Ye Y, Guo M, Ren Q, Liu L, Zhang H, Xu C, Zhou Q, Huang S*, Chen L*. Activation of AMPK and inactivation of Akt results in suppression of mTOR-mediated S6K1 and 4E-BP1 pathways leading to neuronal cell death in in vitro models of Parkinson’s disease. Cell Signal. 2014; 26: 1680-1689 (SCI收录, IF=4.471).
  10. Liang D1, Zeng Q1, Xu Z1, Zhang H, Gui L, Xu C, Chen S, Zhang S, Huang S*, Chen L*. BAFF activates Erk1/2 promoting cell proliferation and survival by Ca2+-CaMKII-dependent inhibition of PP2A in normal and neoplastic B-lymphoid cells. Biochem Pharmacol. 2014; 87: 332-343 (SCI收录, IF=4.65).
  11. Chen S1, Gu C1, Xu C, Zhang J, Xu Y, Ren Q, Guo M, Huang S*, Chen L*. Celastrol prevents cadmium-induced neuronal cell death via targeting JNK and PTEN-Akt/mTOR network. J Neurochem. 2014; 128: 256-266 (SCI收录, IF=4.244).
  12. Ke Z1, Liang D1, Zeng Q, Ren Q, Ma H, Gui L, Chen S, Guo M, Xu Y, Gao W, Zhang S, Chen L*. hsBAFF promotes proliferation and survival in cultured B lymphocytes via calcium signaling activation of mTOR pathway. Cytokine 2013; 62: 310-321 (SCI收录, IF=3.019).
  13. Zhang LJ, Xue YQ, Yang C, Yang WH, Chen L, Zhang QJ, Qu TY, Huang S, Zhao LR, Wang XM, Duan WM. Human albumin prevents 6-hydroxydopamine-induced loss of tyrosine hydroxylase in in vitro and in vivo. PloS ONE 2012; 7: e41226 (SCI收录, IF=4.092).
  14. Han X, Xu B, Beevers CS, Odaka Y, Chen L, Liu L, Luo Y, Zhou H, Chen W, Shen T, Huang S. Curcumin inhibits protein phosphatases 2A and 5, leading to activation of mitogen-activated protein kinases and death in tumor cells. Carcinogenesis. 2012; 33: 868-875 (SCI收录, IF=5.402).
  15. Chen L1, Xu B1, Liu L, Luo Y, Zhou H, Chen W, Shen T, Han X, Kontos CD, Huang S. Cadmium induction of reactive oxygen species activates mTOR pathway, leading to neuronal cell death. Free Radic Biol Med. 2011; 50: 624-632 (SCI收录, IF=5.707).
  16. Xu B1, Chen S1, Luo Y, Chen Z, Liu L, Zhou H, Chen W, Shen T, Han X, Chen L*, Huang S*. Calcium signaling is involved in cadmium-induced neuronal apoptosis via induction of reactive oxygen species and activation of MAPK/mTOR network. PloS ONE 2011; 6: e19052 (SCI收录, IF=4.411).
  17. Chen S1, Xu Y1, Xu B1, Guo M, Zhang Z, Liu L, Ma H, Chen Z, Luo Y, Huang S*, Chen L*. CaMKII is involved in cadmium activation of MAPK and mTOR pathways leading to neuronal cell death. J Neurochem. 2011; 119: 1108-1118 (SCI收录, IF=4.337).
  18. Wen L1, Chen SJ1, Zhang W, Ma HW, Zhang SQ, Chen L*. hsBAFF regulates proliferation and response in cultured CD4+ T lymphocytes by upregulation of intracellular free Ca2+ homeostasis. Cytokine 2011; 53: 215-222 (SCI收录, IF=3.537).
  19. Liu L, Luo Y, Chen L, Shen T, Xu B, Chen W, Zhou H, Han X, Huang S. Rapamycin inhibits cytoskeleton reorganization and cell motility by suppressing RhoA expression and activity. J Biol Chem. 2010; 285: 38362-38373 (SCI收录,  IF=5.328).
  20. Liu L, Chen L, Luo Y, Chen W, Zhou H, Xu B, Han X, Shen T, Huang S. Rapamycin inhibits IGF-1 stimulated cell motility through PP2A pathway. PloS ONE 2010; 5: e10578 (SCI收录, IF=4.351).
  21. Chen L, Huang S. Signaling pathways involved in cadmium-induced neuronal apoptosis. In S. Huang edit: Metals and Neurodegeneration. Research Signpost, Kerala, India. 2010; pp101-121.
  22. Chen L1, Xu B1, Liu L, Luo Y, Yin J, Zhou H, Chen W, Shen T, Han X, Huang S. Hydrogen peroxide inhibits mTOR signaling by activation of AMPKα leading to apoptosis of neuronal cells. Lab Invest. 2010; 90:762-773 (SCI收录, IF=4.602).
  23. Gao W, Chen L*, Xu LB, Huang XH. Specific IgG activity against diarrheagenic bacteria in bovine immune milk and effect of pH on its antigen-binding activity upon heating. J Dairy Res. 2010; 77: 220-224 (SCI收录, IF=1.343).
  24. Chen L, Liu L, Yin J, Luo Y, Huang S. Hydrogen peroxide-induced neuronal apoptosis is associated with inhibition of protein phosphatase 2A and 5, leading to activation of MAPK pathway. Int J Biochem Cell Biol. 2009; 41: 1284-1295 (SCI收录, IF=4.178).
  25. Chen L*, Ran MJ, Shan XX, Cao M, Cao P, Yang XM, Zhang SQ. BAFF enhances B-cell-mediated immune response and vaccine-protection against a very virulent IBDV in chickens. Vaccine 2009; 27: 1393-1399 (SCI收录, IF=3.298).
  26. Beevers CS, Chen L, Liu L, Luo Y, Webster NJ, Huang S. Curcumin disrupts the Mammalian target of rapamycin-raptor complex. Cancer Res. 2009; 69: 1000-1008 (SCI收录, IF=7.514).
  27. Liang JQ, Zhang W, Wen L, Gao W, Zhang SQ, Chen L*. hsBAFF-upregulated intracellular free Ca2+ homeostasis regulates ERK1/2 activity and cell proliferation in in vitro B cells. Physiol Res. 2009; 58: 411-418 (SCI收录, IF=1.658).
  28. Chen L, Liu L, Luo Y, Huang S. Cadmium activates the mitogen-activated protein kinase (MAPK) pathway via induction of reactive oxygen species and inhibition of protein phosphatases 2A and 5. Free Radic Biol Med. 2008; 45: 1035-1044 (SCI收录, IF=5.399).
  29. Chen L, Liu L, Luo Y, Huang S. MAPK and mTOR pathways are involved in cadmium-induced neuronal apoptosis. J Neurochem. 2008; 105: 251-261 (SCI收录, IF=4.5).
  30. Liu L, Chen L, Chung J, Su B, Blenis J, Huang S. Rapamycin inhibits F-actin reorganization and phosphorylation of focal adhesion proteins. Oncogene 2008; 27: 4998–5010 (SCI收录, IF=6.44).
  31. Huang XH, Chen L*, Gao W, Zhang W, Chen SJ, Xu LB, Zhang SQ. Specific IgG activity of bovine immune milk against diarrhea bacteria and its protective effects on pathogen-infected intestinal damages. Vaccine 2008; 26: 5973–5980 (SCI收录, IF=3.377).
  32. Zhang W, Wen L, Huang XH, Liang JQ, Gao W, Zhang SQ, Chen L*. hsBAFF enhances activity of NK cells by regulation of CD4+ T lymphocyte function. Immunol Lett. 2008; 120: 96-102 (SCI收录, IF=2.623).
  33. Xia SK, Chen L*, Liang JQ. Enriched selenium and its effects on growth and biochemical composition in Lactobacillus Bulgaricus. J Agric Food Chem. 2007; 55: 2413-2417 (SCI收录, IF=2.322).
  34. Wang SS, Chen L*, Xia SK. Cadmium is acutely toxic for murine hepatocytes: effects on intracellular free Ca2+ homeostasis. Physiol Res. 2007; 56: 193-201 (SCI收录, IF=2.006).
  35. Xu LB, Chen L*, Gao W, Du KH. Bovine immune colostrum against seventeen strains of diarrhea bacteria and in vitro and in vivo effects of its specific IgG. Vaccine 2006; 24: 2131-2140 (SCI收录, IF=2.87).
  36. Shan X, Chen L*, Cao M, Xu L, Zhang S. Effects of human soluble BAFF synthesized in Escherichia coli on CD4+ and CD8+ T lymphocytes as well as NK cells in mice. Physiol Res. 2006; 55: 301-307 (SCI收录, IF=1.806).
  37. Cao M, Chen L*, Shan XX, Zhang SQ. Immunological effects of refolded human soluble BAFF synthesized in Escherichia coli on murine B lymphocytes in vitro and in vivo. Jpn J Physiol. 2005; 55: 225-231 (SCI收录, IF=0.87).

ö  发明专利

  1. 陈龙, 高伟, 许龙兵. 一种抗婴幼儿腹泻病原菌免疫乳制品及其生产方法. 国家发明专利,授权号CN1306957,专利号200510039259.8;
  2. 陈龙. 一种富硒乳酸菌制剂及其生产方法和应用. 国家发明专利,授权号CN100493520C,专利号200510039258.3。

通讯地址、电话和email信箱

江苏省南京市栖霞区文苑路1号,南京师范大学 生命科学学院 生物化学与生物制品研究所,邮编:210023。

电话:86-25-85891797;Email:lchen@njnu.edu.cn

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